Monodisperse carboxy silica microspheres are widely used in biomedical research for applications such as drug delivery, diagnostics, and bioseparation. Their uniform size and carboxyl functionalization make them ideal for binding biomolecules like proteins, antibodies, or DNA, enabling precise targeting and detection in assays. In chromatography, they serve as stationary phases for efficient separation of complex mixtures due to their high surface area and stability. Additionally, they are employed in imaging and diagnostics as contrast agents or markers due to their biocompatibility and tunable surface properties. These microspheres are also utilized in material science for creating advanced composites or coatings with enhanced mechanical and optical properties.