Monodisperse amino silica microspheres are widely used in chromatography as stationary phases due to their uniform size and surface functionalization. Their amino groups enable strong interactions with polar compounds, making them ideal for separating biomolecules like proteins, peptides, and nucleic acids. In drug delivery systems, these microspheres serve as carriers for controlled release of therapeutic agents, leveraging their biocompatibility and tunable surface properties. They are also employed in catalysis, where the amino groups act as active sites or anchors for immobilizing catalysts, enhancing reaction efficiency. Additionally, their use in diagnostics is notable, particularly in immunoassays and biosensors, where they facilitate the immobilization of biomolecules for sensitive detection. In environmental applications, these microspheres are utilized for the adsorption and removal of heavy metals and organic pollutants from water, owing to their high surface area and functional group reactivity.