ML385 is primarily used in scientific research to inhibit the Nrf2 signaling pathway, a key regulator of cellular antioxidant responses. By selectively blocking Nrf2 activity, ML385 helps researchers study oxidative stress mechanisms and cellular defense systems. It has been applied in cancer research to sensitize tumor cells that overexpress Nrf2, making them more vulnerable to chemotherapeutic agents. Additionally, ML385 is used to investigate inflammatory diseases, neurodegenerative disorders, and conditions where oxidative damage plays a role, offering insights into potential therapeutic strategies targeting redox regulation.