ML335 is primarily used in scientific research as a selective activator of the NLRP3 inflammasome pathway. It plays a key role in studying inflammatory responses, particularly in models of immune cell activation. Researchers apply ML335 to investigate the mechanisms underlying inflammatory diseases such as gout, type 2 diabetes, and neurodegenerative disorders where NLRP3 activation is implicated. Due to its ability to induce caspase-1 activation and interleukin-1β release, ML335 serves as a tool compound for probing inflammasome function in both cellular and animal models. It is not used clinically but supports the development of therapies targeting chronic inflammation.