Used in research to activate protein kinase G (PKG) in cellular signaling studies. It serves as a cell-permeable analog of cyclic GMP (cGMP), making it valuable for investigating pathways related to smooth muscle relaxation, platelet aggregation, and neuronal signaling. Commonly applied in studies of cardiovascular function, inflammation, and cancer cell proliferation due to its ability to mimic endogenous cGMP effects with increased stability. Also utilized in ophthalmic research for exploring intraocular pressure regulation, as well as in investigations of cell death mechanisms (apoptosis).