Used in cell culture applications requiring a serum-free environment, this medium supports the growth and maintenance of MDBK (Madin-Darby Bovine Kidney) cells without the need for animal-derived serum. It is particularly useful in virology studies, vaccine production, and viral antigen expression, where consistent and defined culture conditions are critical. The absence of serum reduces variability between batches and minimizes the risk of contamination with adventitious agents, making it ideal for industrial and research-scale bioprocessing. It also facilitates downstream protein purification by eliminating interference from serum proteins.