H-top culture medium is widely used in advanced cell culture applications, particularly for the expansion and maintenance of stem cells and organoids. It supports high-efficiency cell growth while preserving cellular differentiation potential, making it ideal for regenerative medicine research and drug development studies. The medium is optimized to work under serum-free conditions, ensuring reproducible results and reducing variability in experimental outcomes. It is commonly used in 3D culture systems to grow intestinal, hepatic, and neural organoids, enabling disease modeling and personalized medicine approaches. Its formulation enhances cell viability and function, supporting long-term cultures with minimal stress or genetic instability.