Y-25130 HCl is primarily used in research settings to study its effects on serotonin receptors, particularly the 5-HT3 subtype. It serves as a selective antagonist, helping scientists understand the role of these receptors in various physiological and pathological processes. This compound is valuable in exploring potential therapeutic applications for conditions like nausea, vomiting, and anxiety disorders, where 5-HT3 receptor modulation is relevant. Additionally, it aids in investigating the mechanisms of serotonin signaling in the central and peripheral nervous systems.