BRL-54443 is primarily utilized in research settings to study its effects on serotonin receptors, particularly the 5-HT1E and 5-HT1F subtypes. It is often employed in pharmacological studies to investigate the role of these receptors in various physiological processes, including neurotransmission and vascular function. Researchers use BRL-54443 to explore potential therapeutic applications, such as the treatment of migraines, due to its ability to modulate serotonin pathways. Additionally, it serves as a valuable tool in understanding the mechanisms underlying serotonin-related disorders and in the development of new drugs targeting these receptors.