RP-64477 is primarily used in research settings as a potent and selective antagonist for the 5-HT6 receptor, making it a valuable tool compound in neuroscience and pharmacology. Its main application lies in studying cognitive functions, memory processes, and neurodegenerative disorders such as Alzheimer's disease. Due to its high selectivity, it helps researchers dissect the role of 5-HT6 receptors in modulating neurotransmitter systems like dopamine and acetylcholine. It has also been explored for its potential in improving cognitive deficits and has served as a lead structure in the development of novel therapeutics targeting central nervous system disorders. Its use remains restricted to preclinical research, with no current clinical applications approved.