Bufotenidine is primarily used in scientific research, particularly in pharmacological and neurochemical studies. It acts as a potent agonist at serotonin receptors, especially the 5-HT3 receptor subtype, making it valuable for investigating ion channel function and neural signaling pathways. Due to its structural similarity to serotonin and bufotenin, it is employed to study the effects of tryptamine derivatives on the central nervous system. Its ability to influence neurotransmitter release and neuronal excitability allows researchers to explore mechanisms related to nausea, vomiting, and potential psychoactive effects. Because of its high potency and selectivity, bufotenidine is also used as a reference compound in receptor binding assays and electrophysiological experiments. Its application remains limited to laboratory settings due to its toxicity and lack of therapeutic approval.