U-101017 is primarily utilized in research settings as a selective antagonist for certain serotonin receptors. Its application is significant in neuroscience studies, particularly in understanding the role of serotonin in various physiological and behavioral processes. Researchers employ this compound to investigate receptor-specific mechanisms, aiding in the development of potential therapeutic agents for conditions like anxiety, depression, and other mood disorders. Additionally, U-101017 is used in pharmacological experiments to explore the interactions between serotonin receptors and other neurotransmitter systems, providing insights into complex brain functions and potential drug targets.