SB-224289 hydrochloride is primarily used in research settings to study the role of 5-HT1B receptors in the brain. It acts as a selective antagonist for these receptors, helping scientists understand their function in regulating serotonin levels. This compound is particularly valuable in exploring conditions like depression, anxiety, and addiction, as 5-HT1B receptors are implicated in mood regulation and behavioral responses. Additionally, it aids in investigating the receptor's role in neurotransmission and its potential as a target for therapeutic drug development.