WAY-100635 is primarily used in scientific research to study the serotonin system in the brain. It acts as a selective antagonist for the 5-HT1A receptor, which is involved in regulating mood, anxiety, and other neurological functions. Researchers use it to investigate the role of serotonin receptors in various psychiatric and neurological disorders, such as depression, anxiety, and schizophrenia. It is also employed in positron emission tomography (PET) imaging studies to visualize and quantify 5-HT1A receptor distribution in the brain, aiding in the understanding of receptor dynamics and potential therapeutic targets. Additionally, WAY-100635 helps in evaluating the efficacy of new drugs targeting serotonin pathways by blocking 5-HT1A receptors and observing the resulting physiological effects. Its application is largely confined to preclinical and experimental settings, contributing to advancements in neuroscience and pharmacology.