Methiothepin maleate is primarily utilized in pharmacological research as a serotonin receptor antagonist. It is extensively used in studies to understand the role of serotonin in various physiological and pathological processes. Researchers employ this compound to block serotonin receptors, particularly 5-HT1 and 5-HT2 subtypes, to investigate their involvement in mood regulation, anxiety, and depression. Additionally, it is used in experimental models to explore the impact of serotonin on cardiovascular function, gastrointestinal motility, and central nervous system disorders. Methiothepin maleate also serves as a tool in neuropharmacology to dissect serotonin-mediated signaling pathways and their potential therapeutic targets. Its application extends to behavioral studies, where it helps elucidate the influence of serotonin on animal behavior and cognitive functions.