CP 94253 hydrochloride is primarily used in research settings to study serotonin receptors, particularly the 5-HT1B subtype. It acts as a selective agonist, helping scientists understand the role of these receptors in various physiological processes, such as neurotransmitter release, mood regulation, and vascular function. This compound is also valuable in investigating potential therapeutic targets for conditions like anxiety, depression, and migraines, where serotonin signaling plays a critical role. Additionally, it is utilized in pharmacological studies to explore drug interactions and receptor binding mechanisms.