Prolyl Endopeptidase Inhibitor 1 is primarily used in research to study the role of prolyl endopeptidase in various biological processes. It is particularly valuable in neuroscience, where it helps investigate the enzyme's involvement in cognitive functions and neurodegenerative diseases. By inhibiting prolyl endopeptidase, researchers can explore its impact on memory, learning, and behavior, providing insights into conditions like Alzheimer's disease. Additionally, this inhibitor is utilized in biochemical assays to understand the enzyme's mechanism of action and its interaction with substrates. Its application extends to drug development, where it serves as a tool for designing potential therapeutic agents targeting prolyl endopeptidase-related disorders.