Used primarily as a serine protease inhibitor, it effectively blocks enzymes like trypsin, thrombin, and plasmin, making it valuable in biochemical research for studying enzyme mechanisms and protein interactions. It is also employed in the purification of proteins, particularly in affinity chromatography, where it binds specifically to serine proteases, aiding in their isolation. Additionally, it finds application in medical research for investigating blood coagulation pathways and developing potential therapeutic agents targeting protease-related diseases. Its role in inhibiting proteolytic activity is crucial in cell culture studies to prevent protein degradation.