This compound is widely used in biochemical research as a protease inhibitor and substrate. It effectively inhibits serine proteases, making it valuable in studying enzyme kinetics and mechanisms. Researchers utilize it to analyze protease activity in various biological samples, aiding in understanding disease pathways involving proteolytic enzymes. Additionally, it serves as a substrate in assays to measure protease activity, helping to identify and characterize new protease inhibitors. Its application extends to drug discovery, where it is used to screen potential therapeutic agents targeting proteases implicated in conditions like cancer, inflammation, and infectious diseases. Its specificity and reactivity make it a critical tool in enzymology and biomedical research.