This compound is widely used in biochemical research as an irreversible inhibitor of serine proteases, such as trypsin and plasmin. It effectively blocks the active site of these enzymes, making it valuable for studying protease function and mechanisms in various biological processes. Additionally, it is employed in the development of enzyme inhibitors and in understanding protease-related pathways in diseases like cancer and inflammation. Its role in controlling proteolytic activity also aids in protein purification and structural studies.