T1N1 Agar is widely used in microbiology for the selective isolation and cultivation of thermophilic actinomycetes, particularly in environmental and industrial research. It provides a nutrient-rich medium that supports the growth of heat-tolerant microorganisms when incubated at elevated temperatures. The formulation favors the development of specific bacterial colonies while suppressing others, making it useful in screening for antibiotic-producing strains. It is commonly employed in soil microbiology studies and in the discovery of novel bioactive compounds. Due to its selective properties, T1N1 Agar is also applied in the analysis of compost, hot springs, and other high-temperature environments where thermophilic microbes thrive.