Tergitol-7 Agar is widely used in microbiology for the selective isolation and detection of coliform bacteria, particularly Escherichia coli, in water, food, and dairy samples. It works by inhibiting the growth of gram-positive bacteria and other non-coliform organisms, allowing only gram-negative bacteria to grow. The inclusion of specific indicators enables differentiation of lactose-fermenting coliforms, which appear as distinct colonies due to color changes in the medium. This makes Tergitol-7 Agar a valuable tool in environmental monitoring and quality control testing where rapid and accurate identification of fecal contamination is critical.