Raka-Ray Medium is primarily used in microbiological laboratories for the cultivation and isolation of fastidious microorganisms, particularly those involved in respiratory tract infections. It supports the growth of bacteria such as Haemophilus influenzae and Neisseria species by providing essential nutrients including hemin and nicotinamide adenine dinucleotide (NAD). The medium is often used in clinical diagnostics to identify pathogenic organisms from patient specimens like sputum, throat swabs, and bronchial aspirates. Its enriched formulation enhances recovery rates of sensitive organisms, making it valuable in both hospital and public health settings. Raka-Ray Medium is also utilized in research studies focusing on bacterial pathogenesis and antimicrobial susceptibility testing when precise growth conditions are required.