MFC Agar is used in microbiology laboratories for the selective detection and enumeration of coliform bacteria, including fecal coliforms, in water samples via the membrane filtration method. It incorporates selective inhibitors and indicators that suppress non-target bacteria while allowing coliforms to form distinctive blue colonies upon incubation at 44.5°C. This medium is essential for water quality assessment in drinking water, recreational waters, wastewater, and environmental monitoring to evaluate bacterial contamination levels.