EF-18 Agar is widely used in microbiology for the selective isolation and enumeration of Enterococcus species from environmental, food, and water samples. It supports the growth of enterococci while inhibiting most gram-negative bacteria and competing gram-positive organisms due to its selective agents. The medium is particularly effective in detecting Enterococcus faecalis and Enterococcus faecium in clinical and non-clinical settings. Its formulation allows for easy differentiation of target colonies based on characteristic color changes, enabling rapid identification. EF-18 Agar is commonly employed in public health monitoring, water quality testing, and food safety analysis to assess fecal contamination and hygiene standards.