Used in microbiology laboratories for the selective isolation and differentiation of Enterococcus species, particularly Enterococcus faecalis and Enterococcus faecium, from clinical and non-clinical samples. The medium supports the growth of enterococci while inhibiting gram-negative bacteria and most other gram-positive organisms through selective agents. It often contains chromogenic substrates that allow for easy identification based on colony color—typically producing blue or purple colonies for Enterococcus species. Commonly applied in clinical diagnostics, food safety testing, and environmental monitoring to detect enterococcal contamination and assess hygiene indicators.