Used primarily in microbiology for the cultivation of Pseudomonas species, especially Pseudomonas aeruginosa. It supports the production of water-soluble pigments such as pyocyanin and pyoverdine, which aid in the identification of these organisms. The medium helps differentiate Pseudomonas from other non-fermenting gram-negative bacteria based on pigment formation and growth characteristics. It is also employed in research settings to study bacterial metabolism, antibiotic susceptibility, and virulence factor expression in Pseudomonas.