Used in agricultural biotechnology to cultivate and maintain Rhizobium bacteria, this medium supports nitrogen fixation by promoting bacterial growth in root nodules of leguminous plants. It enhances the development of biofertilizers, reducing the need for synthetic nitrogen fertilizers. The medium is essential in inoculant production, helping improve soil fertility and crop yield in sustainable farming practices. It is also used in research to study symbiotic nitrogen fixation and bacterial-plant interactions.