Used in microbiology laboratories for the selective isolation and differentiation of pathogenic Vibrio species, particularly Vibrio cholerae and Vibrio parahaemolyticus, from clinical and environmental samples. The medium contains chromogenic substrates that allow distinct colony color development, enabling rapid identification based on enzyme activity specific to each Vibrio species. It is commonly used in food safety testing, water quality monitoring, and clinical diagnostics during outbreaks of cholera or seafood-borne gastroenteritis. The chromogenic properties reduce the need for further biochemical confirmation, speeding up the detection process compared to traditional culture methods.