Used in microbiology to isolate and cultivate Pseudomonas species, particularly Pseudomonas aeruginosa, from clinical, environmental, and pharmaceutical samples. It supports the growth of Pseudomonas while inhibiting gram-positive bacteria and many other gram-negative organisms. The selective agents in the medium, such as antibiotics or dyes, suppress unwanted microbes. Pseudomonas colonies are often identifiable by their characteristic pigmentation—producing green, yellow, or blue-green diffusible pigments (pyocyanin and fluorescein). This makes the medium useful in clinical diagnostics, water testing, and quality control in pharmaceuticals where detection of Pseudomonas contamination is critical.