Calcium 2,6-dioxo-1,2,3,6-tetrahydropyrimidine-4-carboxylate, also known as calcium orotate, serves as a bioavailable source of calcium and orotic acid, a key intermediate in the de novo biosynthesis of pyrimidine nucleotides. It is primarily used as a nutritional supplement to support cellular metabolism, particularly in liver function, cardiovascular health, and athletic performance enhancement. In pharmaceutical applications, it acts as an intermediate for synthesizing pyrimidine-based drugs and nucleotide analogs with potential antiviral or anticancer properties. Additionally, it is utilized in biochemical research to study pyrimidine metabolism and develop novel bioactive compounds.