c-di-AMP is widely used in research related to bacterial signaling and immune response modulation. It plays a crucial role in studying bacterial physiology, particularly in understanding how bacteria regulate their cell wall integrity and respond to stress conditions. In immunology, c-di-AMP is recognized as a potent stimulator of the innate immune system, making it valuable for developing vaccines and adjuvants. It activates pathways such as STING (stimulator of interferon genes), which enhances the body's defense against infections. Additionally, c-di-AMP is explored in cancer immunotherapy research due to its ability to induce strong immune responses against tumors. Its applications extend to biotechnology, where it is used to engineer bacterial strains for improved survival and performance in industrial processes.