Cyclic-di-GMP is widely used in research to study bacterial signaling pathways and biofilm formation. It plays a crucial role in regulating bacterial behavior, such as motility, virulence, and the transition between planktonic and biofilm states. In biotechnology, it is explored for its potential in developing anti-biofilm strategies to combat persistent bacterial infections. Additionally, cyclic-di-GMP is investigated in synthetic biology for designing engineered bacterial systems with controlled behaviors. Its applications also extend to agriculture, where it is studied for its role in plant-microbe interactions, potentially aiding in the development of biocontrol agents to enhance crop resilience.