Used as a buffering agent in biochemical and biological applications due to its zwitterionic nature and good solubility in water. Commonly found in electrophoresis buffers, particularly in SDS-PAGE, where it helps maintain stable pH conditions during protein separation. Also employed in cell lysis buffers and protein purification processes because it minimizes denaturation and supports enzyme stability. Its low interference with spectroscopic measurements makes it suitable for assays involving UV detection. Additionally used in cosmetic formulations as a stabilizer and pH adjuster, offering mildness to skin and compatibility with other ingredients.