The 5X All-In-One MasterMix, combined with the AccuRT Genomic DNA Removal Kit, is primarily used in molecular biology for efficient and accurate reverse transcription and PCR amplification. It simplifies the workflow by integrating reverse transcription, DNA removal, and PCR into a single step, saving time and reducing the risk of contamination. This makes it particularly useful for gene expression analysis, where precise quantification of RNA is critical. The AccuRT component ensures the elimination of genomic DNA contamination, enhancing the reliability of results. It is widely applied in diagnostics, research, and biopharmaceutical development, especially in studies involving RNA viruses, cancer biomarkers, and other RNA-based analyses. Its ready-to-use format and high sensitivity make it a valuable tool for laboratories aiming for consistent and reproducible outcomes.