GSK269962B is primarily utilized in research settings as a potent and selective inhibitor of Rho-associated protein kinase (ROCK). Its application is significant in studying cellular processes such as cytoskeletal reorganization, cell migration, and smooth muscle contraction. Researchers employ this compound to investigate the role of ROCK signaling pathways in various diseases, including hypertension, cardiovascular disorders, and neurodegenerative conditions. Additionally, GSK269962B is used in experimental models to explore its potential therapeutic effects in conditions like glaucoma, where ROCK inhibition may help reduce intraocular pressure. Its specificity makes it a valuable tool for understanding the molecular mechanisms underlying ROCK-mediated cellular functions and for developing targeted therapies.