GSK805 is primarily utilized in research settings as a potent and selective inhibitor of the kinase enzyme ROCK2 (Rho-associated coiled-coil containing protein kinase 2). Its application is significant in studying cellular processes such as cytoskeletal organization, cell migration, and smooth muscle contraction. Researchers employ GSK805 to investigate the role of ROCK2 in various diseases, including cancer, cardiovascular disorders, and neurological conditions. By inhibiting ROCK2, it helps elucidate signaling pathways and potential therapeutic targets. Additionally, GSK805 is used in preclinical studies to evaluate its efficacy in modulating disease progression, particularly in conditions where ROCK2 overexpression or hyperactivity is implicated. Its high selectivity makes it a valuable tool for understanding ROCK2-specific mechanisms in cellular and animal models.