Rhosin hydrochloride is primarily used in research settings to study cell signaling pathways, particularly those involving Rho GTPases. It acts as a specific inhibitor of Rho-associated protein kinase (ROCK), making it valuable for investigating the role of Rho/ROCK signaling in various cellular processes such as cytoskeleton organization, cell migration, and cell adhesion. Its application extends to studies in cancer research, where it helps explore the mechanisms of tumor cell invasion and metastasis. Additionally, it is utilized in neuroscience to examine the role of Rho/ROCK in neuronal regeneration and axon guidance. Rhosin hydrochloride is also employed in cardiovascular research to understand its effects on vascular smooth muscle contraction and blood pressure regulation. Its ability to modulate Rho/ROCK signaling makes it a useful tool for developing potential therapeutic strategies in these areas.