Rho-Kinase-IN-1 is primarily used in research settings to study the role of Rho-associated kinase (ROCK) in various cellular processes. It is a potent inhibitor of ROCK, making it valuable for investigating signaling pathways involved in cell contraction, motility, and cytoskeleton organization. Researchers utilize this compound to explore its potential in treating diseases such as hypertension, cardiovascular disorders, and neurological conditions where ROCK signaling is implicated. Additionally, it aids in understanding the mechanisms of cancer cell invasion and metastasis, as ROCK plays a significant role in tumor progression. Its application extends to studying fibrosis and inflammation, providing insights into therapeutic strategies for these conditions.