Ribocil-C R enantiomer is primarily used in research to study bacterial riboswitches, particularly those involved in the biosynthesis of riboflavin (vitamin B2). It acts as a synthetic ligand that specifically targets and inhibits the FMN riboswitch, which regulates gene expression in bacteria. This makes it a valuable tool for understanding bacterial gene regulation mechanisms and exploring potential antibacterial strategies. Researchers utilize it to develop novel antibiotics by disrupting bacterial metabolic pathways, offering a promising approach to combat antibiotic-resistant strains. Its application is largely confined to laboratory settings for experimental and developmental purposes.