Used extensively as a fluorescent dye in biomedical research, particularly in fluorescence microscopy and flow cytometry. It is employed to label proteins, nucleic acids, and other biomolecules for tracking and visualization in cellular and molecular studies. Its bright fluorescence and photostability make it suitable for long-term imaging experiments. Additionally, it is utilized in immunoassays and fluorescence in situ hybridization (FISH) for detecting specific targets in biological samples. The dye is also applied in the development of biosensors and diagnostic tools, aiding in the detection of diseases and biological processes.