Used extensively in bioorthogonal chemistry for labeling and imaging applications, particularly in live-cell imaging and fluorescence microscopy. Its DBCO group allows for rapid and selective reaction with azide-modified biomolecules, enabling efficient conjugation without the need for catalysts. The PEG4 spacer enhances solubility and reduces steric hindrance, improving binding efficiency. The TAMRA fluorophore provides strong red-orange fluorescence, making it ideal for tracking and visualizing cellular processes. Commonly employed in studies involving protein-protein interactions, nucleic acid labeling, and drug delivery systems. Its stability and biocompatibility make it suitable for in vitro and in vivo applications.