Widely used in bioconjugation and fluorescent labeling applications, this chemical is particularly valuable for tracking biomolecules in cellular imaging. Its high fluorescence quantum yield and photostability make it ideal for super-resolution microscopy and flow cytometry. The reactive NHS ester group enables efficient coupling to primary amines on proteins, peptides, and antibodies, forming stable amide bonds. It is commonly applied in multiplex assays due to its distinct spectral properties, allowing clear differentiation from other fluorophores. Additionally, it is used in diagnostic probes and real-time PCR detection systems where sensitive and precise fluorescence signaling is required.