EHT 1864 2HCl is primarily used in research settings to study cellular processes, particularly those involving Rho family GTPases. It acts as an inhibitor of Rac1, Rac2, and Rac3, which are key regulators of cytoskeletal dynamics, cell migration, and proliferation. This compound is valuable in understanding the role of Rac signaling in cancer, as it helps to elucidate mechanisms of tumor cell invasion and metastasis. Additionally, it is employed in neuroscience research to explore Rac's involvement in neuronal development and synaptic plasticity. Its application extends to investigating inflammatory responses, as Rac proteins play a role in immune cell activation and function. EHT 1864 2HCl serves as a critical tool for dissecting the molecular pathways governed by Rac GTPases in various biological contexts.