Endonuclease KasI is primarily used in molecular biology for DNA manipulation and analysis. It is a restriction enzyme that recognizes specific DNA sequences and cleaves them, making it a valuable tool for genetic engineering. Researchers use it to cut DNA at precise locations, enabling the insertion, deletion, or modification of genetic material. This enzyme is particularly useful in cloning experiments, where it helps create recombinant DNA molecules. Additionally, it aids in the study of gene function and regulation by allowing scientists to analyze DNA fragments. Its specificity and efficiency make it a key component in techniques such as restriction mapping and genome editing.