Endonuclease DpnII is widely used in molecular biology for DNA manipulation and analysis. It is particularly valuable in techniques such as restriction fragment length polymorphism (RFLP) analysis, where it cleaves DNA at specific recognition sites to generate fragments for study. This enzyme is also employed in cloning experiments to digest DNA, enabling the insertion of genes into vectors. Additionally, DpnII plays a crucial role in methylation-sensitive DNA analysis, as it specifically cuts methylated DNA sequences, aiding in epigenetic research. Its precision and specificity make it a key tool in genetic engineering and genomic studies.