Endonuclease BstEII is primarily utilized in molecular biology for DNA manipulation and analysis. It is a restriction enzyme that recognizes specific DNA sequences and cleaves them, making it valuable for genetic engineering and cloning experiments. Researchers use it to cut DNA at precise locations, enabling the insertion or removal of genetic material. This enzyme is also employed in restriction fragment length polymorphism (RFLP) analysis to study genetic variations and mutations. Additionally, it plays a role in constructing recombinant DNA molecules, aiding in the development of genetically modified organisms (GMOs) and the production of proteins for therapeutic or industrial purposes. Its specificity and efficiency make it a crucial tool in advancing biotechnological research and applications.