Endonuclease BstBI is widely utilized in molecular biology for DNA manipulation and analysis. It is particularly valuable in restriction enzyme digestion, where it cleaves DNA at specific recognition sites, enabling the study of genetic material. This enzyme is often employed in cloning experiments to create precise cuts in DNA strands, facilitating the insertion of foreign DNA fragments into vectors. Additionally, it plays a role in genotyping and DNA fingerprinting by generating distinct fragment patterns for identification purposes. Its thermostable nature makes it suitable for applications requiring higher temperatures, such as in certain polymerase chain reaction (PCR) protocols. Overall, it is a critical tool in genetic engineering and biotechnology research.