The FLAG peptide is widely used in molecular biology and biochemistry for protein purification and detection. It is commonly fused to proteins of interest to facilitate their isolation using anti-FLAG antibodies. This tag is particularly useful in affinity chromatography, where it allows for the efficient capture and elution of tagged proteins under mild conditions. Additionally, the FLAG peptide is employed in immunofluorescence and Western blotting to visualize and study protein localization and expression. Its small size and high solubility make it a versatile tool for researchers aiming to study protein function and interactions without significantly altering the native protein structure.