The 3X FLAG peptide is widely used in molecular biology and biochemistry for protein purification and detection. Its high affinity for anti-FLAG antibodies makes it an excellent tag for isolating and identifying recombinant proteins. Researchers often fuse the 3X FLAG sequence to a protein of interest, enabling efficient purification through immunoaffinity chromatography. This tag is particularly useful in studying protein-protein interactions, as it allows for the precise isolation of tagged proteins from complex mixtures. Additionally, the 3X FLAG peptide is employed in Western blotting, immunoprecipitation, and fluorescence microscopy, providing a versatile tool for visualizing and analyzing protein expression and localization in cells. Its small size and minimal interference with protein function further enhance its utility in various experimental setups.